特 异 性： 小鼠IFNγ
检测标本： 细胞培养液、体液、血清和组织匀浆等
检测范围： 31.2pg/ml→2000pg/ml

     本试剂盒应用双抗体夹心酶标免疫分析法测定标本中IFN-r水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入IFN-r抗原、生物素化的抗小鼠IFN-r抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IFN-r呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

Intended use

     This immunoassay kit allows for the specific measurement of total mouse IFN-r concentrations in cell culture supernates, serum, and plasma.

 

Test principle

     This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-r has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-r present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for IFN-r is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-r bound in the initial step. The color development is stopped and the intensity of the color is measured.