ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中层连蛋白(LN)含量。

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中层连蛋白水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入层连蛋白、生物素化的抗大鼠层连蛋白抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的层连蛋白呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

This immunoassay kit allows for the specific measurement of rat Laminin (LN) concentrations in cell culture supernates, serum, and plasma.

Introduction

Laminins (LN)are the major non-collagenous component of the basal lamina, such as those on which cells of an epithelium sit.They are a family of glycoproteins that are an integral part of the structural scaffolding of basement membranes in almost every animal tissue. Laminins are secreted and incorporated into cell-associated extracellular matrices.

Laminins form independent networks and are associated with type IV collagen networks via entactin, and perlecan. They also bind to cell membranes through integrin receptors and other plasma membrane molecules, such as the dystroglycan glycoprotein complex and Lutheran blood group glycoprotein.Through these interactions, laminins critically contribute to cell attachment and differentiation, cell shape and movement, maintenance of tissue phenotype, and promotion of tissue survival.Some of these biological functions of laminin have been associated with specific amino-acid sequences or fragments of laminin.

Dysfunctional structure of one particular laminin, laminin-2, is the cause of some forms of muscular dystrophy. Laminin-2 is composed of an α2, a β1 and a γ1 chains. This laminin s distribution includes the brain and muscle fibers. In muscle, it binds to alpha dystroglycan via the G domain, and via the other end binds to the extracellular matrix. Progeria is the result of a defect in the prolaminin receptor that prevents the cleaving and activation of prolaminin into laminin.

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Laminin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Laminin present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Laminin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Laminin bound in the initial step. The color development is stopped and the intensity of the color is measured.