ELISA法定量测定鸡血清、血浆、细胞培养物上清或其它相关液体中IL-4含量。

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中白介素-4水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入白介素-4抗原、生物素化的抗鸡白介素-4抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白介素-4呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

This immunoassay kit allows for the specific measurement of Chicken Interleukin 4,IL-4 concentrations in cell culture supernates, serum, and plasma.

 

Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes,mast cells and basophils . IL-4 has multiple immune response-modulating functions on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the Th2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivoand in vitro.

The biological effects of IL-4 are mediated by specific cell surface receptor complexes. One type of functional IL-4 receptor complex consists of the IL-4-binding subunit (IL-4 R) and a second chain, designated the common c chain because it has also been identified as a component of the receptor complexes for IL-2, IL-7, IL-9 and IL-15. A second type of functional IL-4 receptor complex, consisting of the IL-4 R and the recently cloned IL-13 R, has also been proposed. Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13.

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for IL-4 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.