本试剂盒应用双抗体夹心酶标免疫分析法测定标本中IFN-γ水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入IFN-γ抗原、生物素化的抗猪IFN-γ抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IFN-γ呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
This immunoassay kit allows for the specific measurement of total porcine IFN-γconcentrations in cell culture supernates, serum, and plasma.
Introduction
Interferon gamma (IFN-gama ) is a multifunctional protein first observed as an antiviral activity in
cultures of Sindbis virus-infected leukocytes stimulated by PHA . Produced by T lymphocytes and natural killer (NK) cells, IFN-γ is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. IFN-γinfluences the class of antibody produced by B cells, upregulates class I and II MHC complex antigens and increases.
the efficiency of macrophage-mediated killing of intracellular parasites. Most of the activities attributed to IFN-γ are believed to be mediated by IFN-γ -induced proteins. The appearance of such proteins is a consequence of IFN-γbinding to a specific receptor that is distinct from the receptor for IFN-αandβ. A receptor for IFN-γ has been identified and its gene localized to chromosome .Apparently the product of a single gene, the receptor shows a high degree of species-specific binding of IFN-γ.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-gama has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-gama present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for IFN-gama is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-gama bound in the initial step. The color development is stopped and the intensity of the color is measured.
