Intended use

This immunoassay kit allows for the specific measurement of Human GnRH concentrations in cell culture supernates, serum, and plasma.

 

Introduction

The vertebrate gonadotropin-releasing hormone (GnRH) is a decapeptide involved in regulating reproduction. FSH and LH are secreted by the basophilic cells of the anterior pituitary in response to the Gonadotropin-releasing hormone (GnRH) produced by the hypothalamus. The release of GnRH from the hypothalamus regulates the production of gonadotropins in the pituitary and these gonadotropins are responsible for gonadal development and growth in vertebrates. In addition to the hypothalamic GnRH (also called GnRH1 or GnRH-I), many vertebrate species have been found to express other GnRH forms. These include a midbrain GnRH (called GnRH-II or GnRH2) and a telencephalic GnRH (called GnRH-III or GnRH3). The function of these non-hypothalamic forms remains unclear.

 

Test principle

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GnRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GnRH present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for GnRH is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GnRH bound in the initial step. The color development is stopped and the intensity of the color is measured.