ELISA法定量测定小鼠血清、血浆或其它相关液体中催乳素(PRL)含量。

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中催乳素(PRL)水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入催乳素(PRL)抗原、生物素化的抗小鼠催乳素(PRL)抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的催乳素(PRL)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

Intended use

This immunoassay kit allows for the specific measurement of Mouse prolactin,PRL concentrations in cell culture supernates, serum and plasma.

Introduction

Prolactin (PRL) is a peptide hormone primarily associated with lactation. In breastfeeding, the infant suckling the teat stimulates the production of prolactin, which fills the breast with milk (lactogenesis) in preparation for the next feed. Oxytocin, a similar hormone, is also released, which triggers milk let-down. Prolactin (PRL) is a polypeptide hormone secreted by anterior pituitary of both male and female. Release of prolactin is controlled by a complex neuroendocrine reflex initiated by a tactile stimulus and regulated by hypothalamic releasing and inhibition factors.

It is synthesised and secreted by lactotrope cells in the adenohypophysis (anterior pituitary gland). It is also produced in other tissues including the breast and the decidua.

Pituitary prolactin secretion is regulated by neuroendocrine neurons in the hypothalamus, most importantly by neurosecretory dopamine neurons of the arcuate nucleus, which inhibit prolactin secretion. Thyrotropin-releasing factor has a stimulatory effect on prolactin release.Vasoactive intestinal peptide and peptide histidine isoleucine help to regulate prolactin secretion in humans, but the functions of these hormones in birds can be quite different

Physiologically, the major function of prolactin is to initiate and maintain lactation and breast tissue development. The effect on the mammary gland by this hormone is minimal however without the prior presence and preparation of breast tissue by estrogen, progestin, corticosteroids and insulin. Plasma levels of this hormone are essentially equivalent for male and non-pregnant females. In pregnancy, plasma prolactin increased progressively to peak concentration at delivery. Nursing causes a further attenuation of prolactin secretion, which may be sustained indefinitely. Neo-natal prolactin concentrations are high, but fall to adult levels by three months of age.

Test principle

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Prolactin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Prolactin present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Prolactin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Prolactin bound in the initial step. The color development is stopped and the intensity of the color is measured.