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预期应用
ELISA法定量测定兔子血清、血浆、细胞培养物上清或其它相关液体中IL-10含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中白细胞介素-10水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入白细胞介素-10抗原、生物素化的抗兔子白细胞介素-10抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的白细胞介素-10呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rabbit IL-10 concentrations in cell culture supernates, serum, and plasma.
Introduction
Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of mouse T helper 2 (TH2) clones that suppressed the production of cytokines by T helper 1 (TH1) clones responding to stimulation by antigen in the presence of monocyte/macrophage antigen-presenting cells. The rabbit homologue of mouse IL-10 was subsequently cloned by cross-hybridization. Rabbit and mouse IL-10 are 81% and 73% identical at the nucleotide and amino acid levels, respectively. Both mouse and rabbit IL-10 are also highly homologous to a previously uncharacterized open reading frame in the Epstein-Barr virus (EBV) genome, BCRF1. The BCRF1 gene product is now designated viral IL-10. In the mouse, IL-10 is produced by TH2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+) and normal B cells. In rabbits, TH0-, TH1-, and TH2-like CD4+ T cell clones, B cell lines derived from patients with AIDS and Burkitt’s lymphoma, activated monocytes and peripheral blood T cells, including TH2-like CD8+ (5), CD4+CD45RA+ naive, CD4+CD45RA- “memory” T cells and bronchogenic carcinoma cells were found to have the capacity to produce IL-10
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for IL-10 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of IL-10 bound in the initial step. The color
development is stopped and the intensity of the color is measured. | 
