ELISA法定量测定小鼠血清、血浆、或其它相关液体中雌激素含量。

 

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中雌激素水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入雌激素抗原、生物素化的抗小鼠雌激素抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的雌激素呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

 

This immunoassay kit allows for the specific measurement of mouse estrogen concentrations in serum, and plasma.

 

Natural estrogens are hormones secreted principally by the ovarian follicles and also by the adrenals, corpus luteum, placenta . Exogenous estrogens (natural or synthetic) elicit, to varying degrees, all the pharmacologic responses usually produced by endogenous estrogens. Estrogenic hormones are secreted at varying rates during the menstrual cycle throughout the period of ovarian activity. The gonadotropins of the anterior pituitary regulate secretion of the ovarian hormones, estradiol and estrogen; hypothalamic control of pituitary gonadotropin production is in turn regulated by plasma concentrations of the estrogens and estrogen. During pregnancy, the placenta becomes the main source of estrogens. At the menopause, ovarian secretion of estrogens declines at varying rates.

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This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for estrogen has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any estrogen present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for estrogen is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of estrogen bound in the initial step. The color development is stopped and the intensity of the color is measured.