预期应用
ELISA法定量测定人血清、血浆或其它相关液体中ANG-1含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中ANG-1水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入ANG-1、生物素化的抗ANG-1抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的ANG-1呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human ANG-1 concentrations in serum and plasma.
Introduction
Angiopoietin-1 (Ang1) and Angiopoietin-2 (Ang2) are ligands that regulate tyrosine phosphorylation of the Tie2/Tek receptor on endothelial cells. Proper regulation of Tie2/Tek is required for normal vascular development, by regulating vascular remodeling and endothelial cell interactions. Antiopoietin-2 (Ang2) is an antagonist of Ang1 that competes for binding of the Tie2 receptor and blocks Ang-1 induced Tie2 phosphorylation during vasculogenesis.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for ANG-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG-1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for ANG-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substhumane solution is added to the wells and color develops in proportion to the amount of ANG-1 bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
