预期应用
ELISA法定量测定人血清、血浆或其它相关液体中SCCA2含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中SCCA2水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入SCCA2抗原、生物素化的抗人SCCA2抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的SCCA2呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human SCCA2 concentrations in serum and plasma.
Introduction
Squamous cell carcinoma antigen (SCCA), a member of the serine protease inhibitor (serpin) family, is a subfraction of the tumor associated antigen TA-4, is a glycoprotein, with a molecular weight between 42 to 48 kDa. SCCA2 is a serological marker for squamous cell carcinomas (SCC). Elevated serum levels are correlated with the clinical stage of the disease.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for SCCA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCCA2 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for SCCA2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCCA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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