本试剂盒应用双抗体夹心酶标免疫分析法测定标本中Collagen Type Ⅲ水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Collagen Type Ⅲ抗原、生物素化的抗人Collagen Type Ⅲ抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Collagen Type Ⅲ呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human Collagen type Ⅲ concentrations in cell culture supernates, serum, and plasma.
Introduction
Type III Collagen is found co-localized with Type I Collagen in tissues, blood vessels and skin. Type III Collagen is a homotrimeric procollagen comprised of three identical pro a, (III) chains.
1 Collagen III is important for the development of skin and the cardiovascular system and for maintaining normal physiological functions in adult life. Collagen III has been used for studies of Collagen I fibrillogenesis in normal cardiovascular development. Many of the same properties and functions of animal derived collagens can be duplicated using recombinant human collagens.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Collagen type Ⅲ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Collagen type Ⅲ present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Collagen type Ⅲ is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Collagen type Ⅲ bound in the initial step. The color development is stopped and the intensity of the color is measured.
