预期应用
ELISA法定量测定人血清、血浆或其它相关液体中IV型胶原(Col IV)含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中IV型胶原水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入IV型胶原抗原、生物素化的抗人IV型胶原抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IV型胶原呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human Collagen Type IV,Col IV concentrations in cell culture supernates, serum, and plasma.
Introduction
Collagen type IV is a major component of basement membranes, such as those associated with capillaries and within glomeruli. It is composed of α1(IV) chain and α2(IV) chain in 2:1 ratio. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Collagen type IV has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Collagen type IV present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Collagen type IV is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Collagen type IV bound in the initial step. The color development is stopped and the intensity of the color is measured.
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