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预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中PTH含量。
概述
甲状旁腺激素由84个氨基酸残基组成，分子量为9500，是由甲状旁腺主细胞分泌。主要靶器官为肾和骨，其主要生理功能有促进骨质溶解，动员骨钙入血，血钙增高，骨和血中碱性磷酸酶活力增加；抑制肾小管对磷的吸收。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中PTH水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入PTH抗原、生物素化的抗人PTH抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的PTH呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat PTH in cell culture supernates, serum, and plasma.
Introduction
Rat intact parathyroid hormone (PTH) is an 84 amino acid polypeptide produced by the parathyroid gland with its biological activity residing in the N-terminal region of the peptide. PTH plays an important role in maintaining the concentration of ionized calcium within the limits needed to achieve normal metabolic functions. When serum calcium levels are decreased the parathyroid gland increases secretion of the hormone which results in increased mobilization of calcium from skeletal reserves into the circulation. When levels of serum calcium are increased the secretion of PTH is reduced.
The similarities between rat and human physiology relative to calcium metabolism make rat an excellent live-animal model for studying human skeletal disease and in the pre-clinical evaluation of pharmacologic agents that may alter bone remodeling. Quantitation of only full-length biologically active rat intact PTH with this kit can provide a precise and sensitive assessment of changes in bone and mineral metabolism.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for Adiponectin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Adiponectin present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for Adiponectin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of Adiponectin bound in the initial step. The color
development is stopped and the intensity of the color is measured. | 
