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预期应用
ELISA法定量测定大鼠血清、血浆、尿液、细胞培养物上清或其它相关液体中CTGF含量。
概述
结缔组织生长因子(CTGF),是一种富含半胱氨酸的分泌多肽，与细胞的生长，增生，分泌有关。大鼠结缔组织生长因子是一类能够调节机体有关组织生长的细胞因子，因而在肌体的各类创伤修复过程中具有重要的生理学意义。CTGF基因属即刻早期基因，启动子含转化生长因子B应答元件,可被TGF-B选择性诱导激活,并作为其下游介质促进胶原合成，因此也是一种新的可刺激成纤维细胞增殖和分泌胶原的生长因子。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中CTGF水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CTGF抗原、生物素化的抗大鼠CTGF抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CTGF呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat connective tissue growth factor (CTGF) concentrations in serum and plasma.
Introduction
CTGF is a member of an emerging family of regulatory proteins grouped together under the acronym CCN (CTGF, Cysteine-rich protein (CyR61), and Nephroblastoma overexpression gene (Nov)). Its overexpression is well described in various fibrotic disorders including scleroderma, renal and pulmonary fibrosis, inflammatory bowel disease, and atherosclerosis. CTGF has the ability to affect the ECM metabolism by directly promoting collagen synthesis and regulating the expression of MMPs and TIMPs. Its potential role as a therapeutic target in controlling various ocular fibrotic diseases has been highlighted in recent studies related to corneal scarring,40 ocular cicatricial pemphigoid, and glaucoma filtration surgery. CTGF gene expression had also been demonstrated in rat trabecular meshwork cells, ciliary body, retinal vascular endothelial cells, proliferative vitreoretinopathy membranes, choroidal neovascular membranes, cataractous plaque, corneal scars, tear fluid, and pterygia.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CTGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTGF present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for CTGF is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTGF bound in the initial step. The color development is stopped and the intensity of the color is measured. |
