预期应用
ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中sVCAM-1含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中VCAM-1水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入VCAM-1抗原、生物素化的抗人VCAM-1抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的VCAM-1呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human sVCAM-1 concentrations in cell culture supernates, serum, and plasma.
Introduction
VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1β, IL-4, TNF-α and IFN-γ. VCAM-1 binds to leukocyte integrins VLA-4 and α4β7. The rat and mouse SVCAM-1 proteins share approximately 76% amino acid similarity. VCAM-1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis.
During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/α4β7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for sVCAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sVCAM-1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for sVCAM-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sVCAM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
