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预期应用
ELISA法定量测定人血清、血浆、尿液或其它相关液体中IFABP含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中IFABP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入IFABP抗原、生物素化的抗人IFABP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IFABP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human IFABP concentrations in urine, serum, and plasma.
Introduction
Fatty acid binding proteins (FABP) are small (approximately 15kD) intracellulair proteins with a high degree of tissue specificity. Intestinal FABP (IFABP) is specifically localized in the epithelium cells in the small bowel. Normally IFABP is undetectable in serum. FABP leaks due to its small size rapidly out of damaged cells leading to a rise in blood and urine levels. Many observations indicate that IFABP is a useful biochemical marker for intestinal cell damage in vivo and in vitro. Ischemically damaged cells are characterized histologically by absence (or low presence) of FABP facilitating recognition of areas of ischemically damaged cells.
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