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预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中TXB2含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中血栓素 水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入血栓素 、生物素化的抗大鼠血栓素 抗体、HRP标记的亲和素，经过彻底洗涤后用底物T血栓素 显色。T血栓素 在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血栓素 呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat Motilin concentrations in serum, and plasma.
Introduction
Transport of nutrients through the digestive system is highly dependent on gastrointestinal (GI) motility. Motilin is secreted by enterochromaffin cells of the small intestine, influences gastric motility by inducing interdigestive (phase III) antrum and duodenal contractions. An orphan GPCR related to growth hormone secretagogues receptor (GHS-R) has been identified as the motilin receptor (MTLR or GPR38).
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Motilin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Motilin present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Motilin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Motilin bound in the initial step. The color development is stopped and the intensity of the color is measured.
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