ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中IL-1β含量。

 

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中IL-1β水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入IL-1β、生物素化的抗人IL-1β抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IL-1β呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

This immunoassay kit allows for the specific measurement of total Human IL-1β concentrations in cell culture supernates, serum, and plasma.

 

Interleukin-1 (IL-1) is one of the first cytokines ever described. Its initial discovery was as a factor that could induce fever, control lymphocytes, increase the number of bone marrow cells and cause degeneration of bone joints. At this time, IL-1 was known under several other names including endogenous pyrogen, lymphocyte activating factor, haemopoetin-1 and mononuclear cell factor, amongst others. It was around 1984-1985 when scientists confirmed that IL-1 was actually composed of two distinct proteins, now called IL-1α and IL-1β.These belong to a family of cytokines known as the interleukin-1 superfamily.

Both IL-1α and IL-1β are produced by macrophages, monocytes and dendritic cells. They form an important part of the inflammatory response of the body against infection. These cytokines increase the expression of adhesion factors on endothelial cells to enable transmigration of leukocytes, the cells that fight pathogens, to sites of infection and re-set the hypothalamus thermoregulatory center, leading to an increased body temperature which expresses itself as fever. IL-1 is therefore called an endogenous pyrogen. The increased body temperature helps the body s immune system to fight infection. IL-1 is also important in the regulation of hematopoiesis.

For the most part, these two forms of IL-1 bind to the same cellular receptor. This receptor is composed of two related, but non-identical, subunits that transmit intracellular signals via a pathway that is mostly shared with certain other receptors. These include the Toll family of innate immune receptors and the receptor for IL-18.

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1βhas been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1βpresent is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for IL-1βis added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1βbound in the initial step. The color development is stopped and the intensity of the color is measured.