本试剂盒应用双抗体夹心酶标免疫分析法测定标本中IL-1B水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入IL-1B抗原、生物素化的抗大鼠IL-1B抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IL-1B呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
This immunoassay kit allows for the specific measurement of total rat IL-1B concentrations in cell culture supernates, serum, and plasma.
Introduction
Monocytes are the main source of secreted IL-1. They express predominantly IL-1ß while human keratinocytes express large amounts of IL-1a. IL-1 is produced also by activated macrophages from different sources (alveolar macrophages, Kupffer cells, adherent spleen and peritoneal macrophages) and also by peripheral neutrophil granulocytes. IL-1a and IL-1ß are biologically more or less equivalent pleiotropic factors that act locally and also systemically. IL-1ß is a potent immunomodulator, which mediates a wide range of immune and inflammatory responses including the activation of B and T cells.
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1B present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for IL-1B is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
