预期应用
ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中S-100B含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中S-100B水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入S-100B抗原、生物素化的抗人S-100B抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的S-100B呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human S-100B concentrations in cell culture supernates, serum, and plasma.
Introduction
S-100B is a member of highly homologous Ca2+ binding proteins family that possess two EF-hand motifs. The family of S100 proteins consists of 19 members. Most S100 proteins exist as dimers (frequently homodimers) within cells. Exclusively expressed in vertebrates, S100 is implicated in various intracellular and extracellular regulatory activities. Studies indicate that S100 proteins are involved in the inhibition of protein phosphorylation, inhibition of cytosceletal constituent assembly, regulation of Ca2+ homeostasis, stimulation of enzyme activities, and interaction with transcription factors. S100B is abundant in the nervous system where it is predominantly expressed in astrocytes, oligodendrocytes and Schwann cells. When secreted by astrocytes, S100B has neurotrophic effects during development and nerve regeneration at physiologic (nanomolar) concentrations. However high (micromolar) concentrations of S100B have shown to be neurotoxic, participating in the physiology of neurodegenerative disorders. The clinical values have been demonstrated in stroke, cerebral complications association with cardiac arrest and in patients with severe as well as minor head injury.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for S-100B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S-100B present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for S-100B is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of S-100B bound in the initial step. The color
development is stopped and the intensity of the color is measured.
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