预期应用
ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中I型前胶原羧基端肽(PICP)含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中PICP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入PICP抗原、生物素化的抗大鼠PICP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的PICP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat Carboxyterminal propeptide of type I procollagen,PICP concentrations in cell culture supernates, serum, and plasma.
Introduction
Type I collagen is found in several different types of connective tissues throughout the rat body, but most of it is present in bones, where it forms more than 95% of the organic matrix. Many diseases are associated with an increase in the amount of collagen synthesized i.e. an increase in the rate of bone matrix formation and turnover. In particular it would be desirable to be able to assess this rate since osteoporosis and other metabolic bone diseases are a common health problem and such assessment would provide guidance in formulating a therapy for these disorders.
Type I collagen is synthesized as a procollagen containing propspride extensions at both ends of the molecule.
Cultured skin fibroblasts always produce both type I and III procollagens. However, it is technically difficult to completely separate the two types from each other. Salt fractionation, the most commonly used separation method, does not result in complete separation. The concentration of type III procollagen is often monitored using an assay for the aminoterminal propeptide of type III procollagen. However, this is not sufficiently sensitive since it does not recognize molecules that contain the carboxyterminal propeptide but have lost the aminoterminal one (such molecules are known as type III pC-collagen). Both PICP and PIIICP contain mannose-rich oligosaccharide side chains and they thus bind to concanavalin A lectin.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for PICP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PICP present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for PICP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PICP bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
