本试剂盒应用双抗体夹心酶标免疫分析法测定标本中Ⅰ型胶原水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Ⅰ型胶原抗原、生物素化的抗小鼠Ⅰ型胶原抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Ⅰ型胶原呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of mouse Collagen Type Ⅰ concentrations in cell culture supernates, serum, and plasma.
Introduction
Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Type 1 collagen is the most abundant protein in the connective tissues, especially in tendon, skin and bone.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Collagen Type Ⅰ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Collagen Type Ⅰ present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Collagen Type Ⅰ is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Collagen Type Ⅰ bound in the initial step. The color development is stopped and the intensity of the color is measured.
