ELISA法定量测定大鼠血清、血浆或其它相关液体中PⅢNP含量。

 

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中PⅢNP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入PⅢNP抗原、生物素化的抗大鼠PⅢNP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的PⅢNP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

 

This immunoassay kit allows for the specific measurement of rat procollagen type III peptide (PⅢNP) concentrations in serum and plasma.

 

Different kinds of collagen have been identified in rats. All of them derive from longer precursor molecules (procollagens). They are synthesized intracellularly and secreted in extracellular space where they are cleaved by aminoproteases (1-3). Among the different kinds of precursors, type III is one of the most abundant interstitial procollagens. Since its aminoterminal propeptide, PIIIP, is formed in equimolar proportions to collagen, serum measurements of this fragment can provide an index of collagen synthesis (3).

1.Prockop DJ, Kivirikko KI, Tuderman L, Guzman NA: The biosynthesis of collagen and its disorders. N Engl J Med 1979, 301:13-23.
2.Querejeta R, Varo N, Lopez B, Larmanet M, Artinano E, Etayo JC, Martinez JL, Gutierrez-Stampa M, Emparanza JI, Gil MJ, Monreal I, Mindan JP, Diez J: Serum carboxi-terminal propeptide of procollagen type I is a marker of myocardial fibrosis in hypertensive heart disease. Circulation 2000, 101:1729-1735.
3. Fessler JH, Fessler LI: Biosynthesis of procollagen. Annu Rev Biochem 1978, 47:129-162.

 

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for PⅢNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PⅢNP present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for PⅢNP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PⅢNP bound in the initial step. The color development is stopped and the intensity of the color is measured.