预期应用

ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中生长抑素含量。

 

实验原理

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中生长抑素水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入生长抑素抗原、生物素化的抗大鼠生长抑素抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的生长抑素呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

 

Intended use

This immunoassay kit allows for the specific measurement of rat Somatostatin concentrations in cell culture supernates, serum, and plasma.

 

Introduction

Somatostatin was first discovered in hypothalamic extracts and identified as a hormone that inhibited secretion of growth hormone. Subsequently, somatostatin was found to be secreted by a broad range of tissues, including pancreas, intestinal tract and regions of the central nervous system outside the hypothalamus. Two forms of somatostatin are synthesized. They are referred to as SS-14 and SS-28, reflecting their amino acid chain length. Both forms of somatostatin are generated by proteolytic cleavage of prosomatostatin, which itself is derived from preprosomatostatin. Two cysteine residules in SS-14 allow the peptide to form an internal disulfide bond.

 

Test principle

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal

antibody specific for Somatostatin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Somatostatin present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Somatostatin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Somatostatin bound in the initial step. The color development is stopped and the intensity of the color is measured.