ELISA法定量测定人血清、血浆或其它相关液体中降钙素基因相关肽(CGRP)含量。

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中CGRP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CGRP抗原、生物素化的抗人CGRP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的CGRP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

This immunoassay kit allows for the specific measurement of human calcitonin gene related peptide,CGRP concentrations in cell culture supernates, serum, and plasma.

Calcitonin Gene Related Peptide is a potent hypotensive agent and a member of the Calcitonin/CGRP multigene complex. This peptide is 37 amino acids long containing one disulfide bridge. It is highly conserved among species indicating its importance as a vasoactive peptide and shares homology with Amylin, Adrenomedullin and Calcitonin. It has been suggested that these peptides may have shared an ancestral gene. CGRP has been shown to elevate the flow of blood and enhance the contractibility of the atrium. CGRP shares some effects of Calcitonin on the osteoclast and renal Calcitonin receptors, but at several hundred fold molar concentration.

CGRP is an important cutaneous neuropeptide. It is widely expressed in both the central and peripheral nervous system. CGRP is known as pro inflammatory factor and is thought to be involved in neurogenic inflammation. It was reported that CGRP stimulate the adhesiveness of human umbilical vein endothelial cells for monocytic cell line and neutrophils.The function of CGRP in skin remains unclear. However, evidences suggested that it may be directly involved in modulating the functions of keratinocytes, Langerhans cells and microvascular endothelial cells in the skin.On the other hand, psoriasis is generally known as inflammatory and proliferative skin disorder. In psoriatic plaque lesion there are epidermal influx of polymorphonulear leukocytes, and the presence of mononuclear infiltration in the papillary dermis and in the epidermis. In order to study the mechanism of stress exacerbating psoriasis and the involvement effect of CGRP in psoriatic pathogenesis.

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal

antibody specific for CGRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CGRP present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for CGRP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CGRP bound in the initial step. The color development is stopped and the intensity of the color is measured.