本试剂盒应用双抗体夹心酶标免疫分析法测定标本中碳酸酐酶水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入碳酸酐酶抗原、生物素化的抗鸡碳酸酐酶抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的碳酸酐酶呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of chicken CA concentrations in serum, and plasma.
Introduction
Carbonic anhydrases are enzymes that catalyze the hydration of carbon dioxide and the dehydration of bicarbonate. Carbonic anhydrase isozymes are metalloenzymes consisting of a single polypeptide chain (Mr ~ 29,000) complexed to an atom of zinc. They are incredibly active catalysts, with a turnover rate (kcat) of about 106 reactions per second! Catalytic activity depends on ionization of a group of pKa 7 and, as you might expect from thinking about when and where the above reactions take place, the hydration reaction depends on the ionized group being in the basic form, and for dehydration in the acidic form.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for CA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CA present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for CA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of CA bound in the initial step. The color
development is stopped and the intensity of the color is measured.
