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预期应用
ELISA法定量测定人血清、血浆或其它相关液体中HSP70含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中HSP70水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入HSP70抗原、生物素化的抗人HSP70抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的HSP70呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human HSP70 concentrations in serum and plasma.
Introduction
Heat shock proteins or HSPs are being synthesized under different kind of stress conditions and act as molecular chaperones for protein molecules. Because these proteins were first found in cells that were exposed to high temperatures， they are called "heat shock proteins" and have been named according to their molecular weights. Usually HSPs are cytoplasmic proteins and they function in various intra-cellular processes. Heat shock proteins play an important role in protein-protein interactions， including folding and assisting in establishing of proper protein conformation， and prevention of inappropriate protein aggregation.
The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses， including heat stress， in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70， a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72， a 72 kDa protein which is induced exclusively under stress conditions; HSC70， or cognate protein， is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP78， or BiP， is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP70 (mtHSP70， GRP75 or mortalin) a 75 kDa protein that is found within the mitochondria.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for HSP70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP70 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for HSP70 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP70 bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
