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预期应用
ELISA法定量测定人血清、血浆或其它相关液体中HSP90含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中HSP90水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入HSP90抗原、生物素化的抗人HSP90抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的HSP90呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human HSP90 concentrations in serum and plasma.
Introduction
Heat shock proteins or HSPs are being synthesized under different kind of stress conditions and act as molecular chaperones for protein molecules. Because these proteins were first found in cells that were exposed to high temperatures， they are called "heat shock proteins" and have been named according to their molecular weights. Usually HSPs are cytoplasmic proteins and they function in various intra-cellular processes. Heat shock proteins play an important role in protein-protein interactions， including folding and assisting in establishing of proper protein conformation， and prevention of inappropriate protein aggregation.
HSP90 has been identified in the cytosol， nucleus and endoplasmic reticulum， and is reported to exist in many tissue types. In several tissues， including smooth muscle， HSP90 comprises up to 2% of total cellular protein. HSP90 functions as a dimer， assembled as part of heterocomplex. It possesses ATP-binding site and low ATPase activity. HSP90 is able to associate with actin filaments in certain conditions. Another important property of HSP90 is the binding of unoccupied steroid hormone receptors. HSP90 has been characterized as a molecular chaperone able to keep the target protein in a folding-competent state. It has an enhanced chaperone activity in oligomeric form at high temperatures. HSP90 function is sensitive to bivalent cations concentration.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for HSP90 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP90 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for HSP90 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP90 bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
