预期应用

    ELISA法定量测定猪血清、血浆或其它相关液体中ANG-2含量。

 

实验原理

    本试剂盒应用双抗体夹心酶标免疫分析法测定标本中ANG-2水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入ANG-2、生物素化的抗ANG-2抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的ANG-2呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

 

Intended use

    This immunoassay kit allows for the specific measurement of porcine ANG-2 concentrations in serum and plasma.

 

Introduction

    Angiopoietin 2 and angiopoietin 1 have an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. The coiled coil domains mediate ligand homo-oligomerization and the fibrinogen-like domains mediate ligand activity. Recombinant human angiopoietin 2 has a molecular mass of approximately 66 kDa in SDS-PAGE under reducing and non-reducing conditions. Angiopoietin 2 probably acts as a natural antagonist for Ang1 and Tie2 both in vivo and in vitro. In adult mice and humans, angiopoietin 2 is expressed at sites of vascular remodeling.

 

Test principle

    This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for ANG-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG-2 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for ANG-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substporcinee solution is added to the wells and color develops in proportion to the amount of ANG-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.