预期应用

    ELISA法定量测定大鼠血清、血浆、尿液、细胞培养物上清或其它相关液体大鼠葡萄糖依赖性胰岛素释放多肽(GIP)中含量。

概述

    GIP是胃肠道的一种组成的多肽激素，又称为葡萄糖依赖性促胰岛素多肽（glucose-dependent insulinotropic peptide，GIP）他强烈抑制胃分泌和运动，也可调节胰岛素的释放。GIP属于Glucagon家族成员。

实验原理

    本试剂盒应用双抗体夹心酶标免疫分析法测定标本中GIP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入GIP抗原、生物素化的抗大鼠GIP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的GIP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

Intended use

    This immunoassay kit allows for the specific measurement of Rat Glucose-dependent insulin-releasing polypeptide (GIP) concentrations in serum and plasma.

 

Introduction

    "Gastric inhibitory peptide" or "glucose-dependent insulin-releasing peptide" (GIP) is a member of the gut hormone family. Its physiological action is thought to be related to its insulinotrophic effect. Gastric inhibitory polypeptide (glucose-dependent insulin-releasing polypeptide) has a similar effect, in particular at concentrations close to the physiological level in blood. The nutrient-dependent glucagon-like peptide-1 amide (GLP-1) release was studied in comparison to the glucose-dependent insulin-releasing polypeptide (GIP) response. GIP was found to reside in the glucagon cells of the pancrease and gut. Three pancreatic glucagonomas were found to contain numerous cells displaying GIP and glucagon immunoreactivity. The GIP antiserum used did not cross react with either pancreatic-type or gut-type glucagon (GLI).

Test principle

    This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GIP present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for GIP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GIP bound in the initial step. The color development is stopped and the intensity of the color is measured.