预期应用

ELISA法定量测定大鼠血清、血浆、细胞培养物上清或其它相关液体中Cytochrome C含量。

 

实验原理

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中Cytochrome C水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Cytochrome C抗原、生物素化的抗大鼠Cytochrome C抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Cytochrome C呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

 

Intended use

This immunoassay kit allows for the specific measurement of rat Cytochrome C concentrations in cell culture supernates, serum, and plasma.

 

Test principle

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal

antibody specific for Cytochrome C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cytochrome C present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Cytochrome C is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cytochrome C bound in the initial step. The color development is stopped and the intensity of the color is measured.