预期应用

ELISA法定量测定犬血清、血浆或其它相关液体中PSA含量。

 

实验原理

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中PSA水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入PSA抗原、生物素化的抗犬PSA抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的PSA呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

Intended use

This immunoassay kit allows for the specific measurement of canine PSA concentrations in serum,and plasma.

 

Introduction

Prostate-specific antigen (PSA) is a serine protease, a single chain glycoprotein with a molecular weight of approximately34,000 daltons containing 7% carbohydrate by weight. PSA is immunologically specific for prostatic tissue, it is present in normal, benign hyperplastic, and malignant prostatic tissue, in metastatic prostatic carcinoma, and also in prostatic fluid and seminal plasma. PSA is not present in any other normal tissue obtained from men, nor is it produced by cancers of the breast, lung, colon, rectum, stomach, pancreas or thyroid. Besides, it is functionally and immunologically different from prostatic acid phosphatase (PAP). Elevated serum PSA concentrations have been reported in patients with prostate cancer, benign prostatic hypertrophy, or inflammatory conditions of other adjacent genitourinary tissues, but not in apparently healthy men, men with non-prostatic carcinoma, apparently healthy women, or women with cancer. Reports have suggested that serum PSA is one of the most useful tumor markers in oncology. It may serves as an accurate marker for assessing response to treatment in patients with prostatic cancer. Therefore, measurement of serum PSA concentrations can be an important tool in monitoring patients with prostatic cancer and in determining the potential and actual effectiveness of surgery or other therapies. Recent studies also indicate that PSA measurements can enhance early prostate cancer detection when combined with digital rectal examination (DRE).

 

Test principle

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal

antibody specific for PSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PSA present is bound by the immobilized antibody. An

enzyme-linked monoclonal antibody specific for PSA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSA bound in the initial step. The color development is stopped and the intensity of the color is measured.