本试剂盒应用双抗体夹心酶标免疫分析法测定标本中PPAR-γ水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入PPAR-γ抗原、生物素化的抗大鼠PPAR-γ抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的PPAR-γ呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat PPAR-γ concentrations in serum, plasma and cell culture supernates.
Introduction
Peroxisome Proliferator-Activated Receptor gamma (PPARγ; NR1C3) is a member of the orphan nuclear receptor family. Oxidized metabolites of linoleic acid, 9-hydroxyctadienoic acid (9-HODE) and 13-HODE are activators and ligands of PPARγ. PPARγ is expressed in white adipose tissue, intestinal mucosa, colon, spleen, monocytes, macrophages, retina, cartilage, osteoclasts, and skeletal muscle. PPARγ plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease. Three known family members are called PPARα, δ, and γ. Three N-terminal isoforms, called γ1, γ2 and γ3, are known to arise by alternative splicing and promoter usage from the PPARγ gene. RXR is an obligate partner for PPAR.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for PPARγ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPARγ present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for PPARγ is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPARγ bound in the initial step. The color development is stopped and the intensity of the color is measured.
