预期应用

ELISA法定量测定人血清、血浆、或其它相关液体中补体C3含量。

 

实验原理

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中补体C3水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入补体C3抗原、生物素化的抗人补体C3抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的补体C3呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

Intended use

This immunoassay kit allows for the specific measurement of human Complement C3 concentrations in serum and plasma.

 

Introduction

Complement C3 is the major plasma proteins of the immune system complement pathways. The synthesis of these proteins is increased in response to inflammation and infection but at a slower rate than for traditional acute phase proteins.

Complement protein C3 is the fourth component to attach in the complement reaction sequence. It is a beta-globulin with a sedimentation coefficient of 5.5 and a molecular weight of 185,000. C3 is a central molecule in the complement system whose activation is essential for all the important functions performed by this system. C3 can promote phagocytosis during an inflammatory response against pathogens, but unregulated activation of C3 could lead to host cell damage. Low level of serum C3 associates with hypocomplementaemia, primary biliary cirrhosis

 

Test principle

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal

antibody specific for Complement C3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Complement C3 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Complement C3 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Complement C3 bound in the initial step. The color development is stopped and the intensity of the color is measured.