预期应用

ELISA法定量测定犬血清、血浆或其它相关液体中肌钙蛋白I（Tn-I）含量。

 

实验原理

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中肌钙蛋白I水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入肌钙蛋白I抗原、生物素化的抗犬肌钙蛋白I抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的肌钙蛋白I呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

 

Intended use

This immunoassay kit allows for the specific measurement of Canine Troponin I (Tn-I) concentrations in serum and plasma.

 

Introduction

Troponin is the inhibitory or contractile regulating protein complex of striated muscle. It is located

periodically along the thin filament of the muscle and consists of three distinct proteins: troponin I, troponin C, and troponin T. Likewise, the troponin I subunit exists in three separate isoforms; two in fast-twitch and slow-twitch skeletal muscle fibers, and one in cardiac muscle.

The most recently described and preferred biomarker for myocardial damage is troponin (I or T). The troponins exhibit myocardial tissue specificity and high sensitivity. The level of Tn-I remains elevated for a much longer period of time (6-10 days), thus providing for a longer window of detection of cardiac injury. Normal levels of Tn -I in the blood are very low. After the onset of an AMI, Tn-I levels increase substantially and are measurable in serum within 4 to 6 hours, with peak concentrations reached in approximately 12 to 24 hours after infarction. The Tn-I Enzyme Immunoassay provides a rapid, sensitive, and reliable assay for the quantitative measurement of specific troponin I.

 

Test principle

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Troponin I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Troponin I present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Troponin I is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Troponin I bound in the initial step. The color development is stopped and the intensity of the color is measured.