本试剂盒应用双抗体夹心酶标免疫分析法测定标本中GnRH水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入GnRH、生物素化的抗鱼GnRH抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的GnRH呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of fish GnRH concentrations in serum and plasma.
Introduction
The vertebrate gonadotropin-releasing hormone (GnRH) is a decapeptide involved in regulating reproduction. FSH and LH are secreted by the basophilic cells of the anterior pituitary in response to the Gonadotropin-releasing hormone (GnRH) produced by the hypothalamus. The release of GnRH from the hypothalamus regulates the production of gonadotropins in the pituitary and these gonadotropins are responsible for gonadal development and growth in vertebrates. In addition to the hypothalamic GnRH (also called GnRH1 or GnRH-I), many vertebrate species have been found to express other GnRH forms. These include a midbrain GnRH (called GnRH-II or GnRH2) and a telencephalic GnRH (called GnRH-III or GnRH3). The function of these non-hypothalamic forms remains unclear.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for GnRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GnRH present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for GnRH is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GnRH bound in the initial step. The color development is stopped and the intensity of the color is measured.
