预期应用
ELISA法定量测定人血清、血浆、细胞培养物上清或其它相关液体中LTF含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中LTF水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入LTF抗原、生物素化的抗人LTF抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的LTF呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of mouse LTF concentrations in cell culture supernates, serum, and plasma.
Introduction
Lactoferrin (LTF) is found in most body fluids and secretions, e.g., in the nose, genital tract, and tears.1 In the blood, LTF is secreted by neutrophils and its plasma concentration is positively related to the total pool of neutrophils and to the rate of neutrophil turnover.2 Because of its ability to strongly bind iron, LTF is considered to be bactericidal.3 In a number of cases of inflammation, LTF is released into the extracellular medium from secondary granules of neutrophilic leukocytes.4,5 Its extracellular concentration can therefore be used as an index of neutrophil activation, especially in blood samples containing anti-myeloperoxidase antibodies.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for LTF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LTF present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for LTF is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LTF bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
