预期应用
ELISA法定量测定小鼠血清、血浆或其它相关液体中己糖激酶(Hexokinase)含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中己糖激酶水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入己糖激酶抗原、生物素化的抗小鼠己糖激酶抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的己糖激酶呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Mouse Hexokinase concentrations in cell culture supernates, serum and plasma.
Introduction
A hexokinase is an enzyme that phosphorylates a six-carbon sugar, a hexose, to a hexose phosphate. In most tissues and organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product.Hexokinases have been found in every organism checked, ranging from bacteria, yeast, and plants to humans and other vertebrates. They are categorized as actin fold proteins, sharing a common ATP binding site core surrounded by more variable sequences that determine substrate affinities and other properties. Several hexokinase isoforms or isozymes providing different functions can occur in a single species.
There are four important mammalian hexokinase isozymes (EC 2.7.1.1) that vary somewhat in their subcellular locations, kinetic characteristics with respect to different substrates and operating conditions, and physiological function. They are designated hexokinases I, II, III, and IV or hexokinases A, B, C, and D.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Hexokinase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hexokinase present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Hexokinase is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hexokinase bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
