预期应用
ELISA法定量测定豚鼠血清、血浆、细胞培养物上清或其它相关液体中Anti-OVA-IgE含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中Anti-OVA-IgE水平。用纯化的OVA包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Anti-OVA-IgE抗原、生物素化的抗豚鼠Anti-OVA-IgE抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Anti-OVA-IgE呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Guine-pig Anti-OVA-IgE concentrations in cell culture supernates, serum, and plasma.
Introduction
Measurement of anti-OVA-IgE levels after OVA-inoculation to mouse will be helpful in clarification of the immune process.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. OVA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Anti-OVA-IgE present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Anti-OVA-IgE is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-OVA-IgE bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
