预期应用
ELISA法定量测定人血浆或其它相关液体中PDGF-BB含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中PDGF-BB水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入PDGF-BB抗原、生物素化的抗人PDGF-BB抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的PDGF-BB呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of human PDGF-BB concentrations in plasma.
Introduction
Platelet-Derived Growth Factor (PDGF) is synthesized mainly by megakaryocytes. It is stored in the alpha granules of platelets from which it is released after cell activation of platelets for example by thrombin. There are two types of polypeptide, A (16 kDa, 124 amino acids) and B (14 kDa, 140 amino acids), with about 50% sequence identity, disulfide linked into three possible dimeric molecules, PDGF-AA, -AB and –BB. PDGF binds to several plasma proteins and also to proteins of the extracellular matrix. In the adult organism PDGF is involved in wound healing processes. The aberrant expression of PDGF is observed with vascular proliferative diseases such as atherosclerosis. PDGF and PDGF-like factors are autocrine growth factor for meningiomas.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for PDGF-BB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGF-BB present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for PDGF-BB is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
