预期应用
ELISA法定量测定裸鼠血清、血浆或其它相关液体中甲胎蛋白(AFP)含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中AFP水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入AFP抗原、生物素化的抗裸鼠AFP抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的AFP呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Nude mouse Alpha-fetoprotein,AFP concentrations in cell culture supernates, serum, and plasma.
Introduction
AFP is a glycoprotein with a molecular weight of between 65,000 and 70,000 daltons including 4% of carbohydrate . During fetal development, AFP maintains high levels in the serum and drops to very low levels throughout the remainder of life. AFP is elevated in the malignant diseases of hepatocellular, testicular nonseminomatous origin, and occasionally of other entodermal origin. AFP may be slightly elevated or persisted in the patients with large hepatic metastases or viral hepatitis. AFP measurement is widely accepted as tumor marker and for monitoring the therapeutic effectiveness of hepatocellular cancer and nonseminomatous testicular cancer .
The determination of alpha-fetoprotein (AFP) plays an important role in pregnancy monitoring. In the first trimenon AFP is predominantly produced in the vitelline (yolk) sac, in the further course of the pregnancy it is produced in the fetal liver and in the fetal gastrointestinal tract. Normally the AFP reaches the amniotic fluid by the fetal urine and from there it reaches the maternal circulation by the placenta. If the spinal canal is incompletely closed as, for example, in the case of splitted (spina bifida) vertebral column, greater amounts of AFP may reach the amniotic fluid and rise the AFP titer in the maternal blood.
In the blood of healthy adults only small amounts of AFP can be detected. A rise of the APF titer in serum may be pathognomonic for different clinical pictures, like, for example, a primary hepatic carcinoma or germ cell malignomes with participation of the vitelline sac or mixed tumours. For these tumours, AFP is the marker of first choice. Slighty increased titers of AFP are pathognomonic for an inflammation. If, during follow-up investigations, a continuous increase is stated a cancerous process is probable.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for AFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AFP present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for AFP is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFP bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
