ELISA法定量测定裸鼠血清、血浆或其它相关液体中COX-2含量。

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中COX-2水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入COX-2抗原、生物素化的抗裸鼠COX-2抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的COX-2呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

This immunoassay kit allows for the specific measurement of Nude mouse COX-2  concentrations in cell culture supernates, serum, and plasma.

Cyclooxygenase (COX) enzymes catalyze the synthesis of prostaglandins (PGs) from arachidonic acid. There are two isoforms of COX, COX-1 and COX-2. While COX-1 is expressed constitutively and appears to be responsible for the production of prostaglandins (PGs) that control normal physiologic functions, expression of COX-2 is induced by various inflammatory and mitogenic stimuli such as cytokines, growth factors or tumor promoters. Numerous experimental studies suggest a relationship between COX-2 expression and carcinogenesis. For example, increased amounts of COX-2 have been observed in breast cancers that overexpress HER-2/neu. Furthermore, treatment with selective inhibitors of COX-2 reduced the formation of tongue, esophagal, intestinal, breast, skin, lung, and bladder tumors in experimental animals.

Cyclooxygenase-2 (COX2) also known as prostaglandin G/H synthase 2 (PGHS2) is a 70 kDa microsomal enzyme that belongs to the prostaglandin G/H synthase family. It is inducibly-expressed by a number of cell types, including fibroblasts, vascular smooth muscle cells, endothelium, and monocytes. Functionally, COX2 is a homodimer that catalyzes two steps in the conversion of arachadonic acid to prostaglandin H2.

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal

antibody specific for COX-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COX-2 present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for COX-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COX-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.