预期应用
ELISA法定量测定大鼠血清、血浆或其它相关液体中内脂素含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中内脂素水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入内脂素抗原、生物素化的抗大鼠内脂素抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的内脂素呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rat visfatin concentrations in serum and plasma.
Summary
Investigators identified an adipokine that is synthesized primarily by visceral fat and termed this molecule “visfatin.” They subsequently found that the visfatin had been previously identified as “pre-B cell colony-enhancing factor” (PBEF). This is secreted by the liver, bone marrow, and muscle, is a growth factor for early stage B lymphocytes, and down-regulates apoptosis of neutrophils.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for visfatin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any visfatin present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for visfatin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of visfatin bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
