预期应用
ELISA法定量测定小鼠血清、血浆、细胞培养物上清或其它相关液体中Ⅰ型胶原N末端肽(NTx)含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中NTx水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入NTx、生物素化的抗小鼠NTx抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的NTx呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Mouse NTx concentrations in cell culture supernates, serum, and plasma.
Introduction
NTx assays are sensitive and specific assays that can be used to indicate subtle changes in levels of bone resorption. NTx serum and urine assays provide a quantitative measurement of the cross-linked N-telopeptides of bone type I collagen (NTx). NTx is a specific biochemical indicator of bone resorption that is generated as a result of osteoclast activity on bone. The NTx molecule is specific to bone due to the unique amino acid sequence and orientation of the cross-linked alpha-2 (I) N-telopeptide.
NTx provides a dynamic indicator of the current level of bone resorption, shows significantly earlier response to therapy (with 3-6 months) and, when used in conjunction with bone mineral density (BMD), gives a more complete picture of bone status than either technology alone.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for NTx has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NTx present is bound by the immobilized antibody. An enzyme-linkedpolyclonal antibody specific for NTx is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NTx bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
