预期应用
ELISA法定量测定鸡血清、血浆以及蛋清和蛋黄中透明质酸（HA）含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中透明质酸水平。用纯化的透明质酸结合蛋白(HABP)包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入透明质酸、生物素化的抗鸡透明质酸结合蛋白（HABP）、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的透明质酸呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of Chicken HA concentrations in yolk,egg white,serum and plasma.
Introduction
Hyaluronic Acid (HA), also called hyaluronate or hyaluronan, is a mucopolysaccharide widely distributed throughout the body. HA is produced mainly by fibroblasts and other specialized connective tissue cells. As a free molecule, HA can be found in the plasma and synovial fluid. HA is quickly removed from circulation by specific receptors present in sinusoidal cells (SEC) of the liver; the estimated half-life in plasma is 5-6 minutes.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A Hyaluronic Acid binding protein (HABP) specific for HA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HA present is bound. An enzyme-linked HABP specific for HA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HA bound in the initial step. The color development is stopped and the intensity of the color is measured. | 
