预期应用
ELISA法定量测定兔血清、血浆、细胞培养物上清或其它相关液体中C3a含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中C3a水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入C3a抗原、生物素化的抗兔C3a抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的C3a呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of rabbit complement fragment C3a concentrations in serum and plasma.
Introduction
C3a is generated during the activation of the complement system via the classical or alternative pathway. The anaphylatoxin C3a itself is very short lived and in serum is cleaved rapidly to the more stable C3a-desArg. Therefore, quantitation of C3a-desArg allows reliable conclusions about the level of complement activation in a test sample.
1. Mold, C, Tamerius, J.D., et al, "Complement activation during painful crisis in sickle cell anemia", Clinical Immunology and Immunopathology, 70:3,314-320 (1995).
2. Sorace, J., et al, "Role of atheroma liposomes and malondialdehyde modified low density lipoproteins in complement activation", Pathobiology, 64:73-78 (1996).
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for C3a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C3a present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for C3a is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of C3a bound in the initial step. The color
development is stopped and the intensity of the color is measured. | 
