预期应用
ELISA法定量测定猪血清、血浆、细胞培养物上清或其它相关液体中BCL-2含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中BCL-2水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入BCL-2抗原、生物素化的抗猪BCL-2抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的BCL-2呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of porcine BCL-2 concentrations in cell culture supernates, serum, and plasma.
Introduction
B-cell leukemia/lymphoma 2 (Bcl-2) was one of the first oncogenic proteins discovered to inhibit apoptosis. Bcl-2 is localized within the outer mitochondrial membrane, endoplasmic reticulum, and the nuclear envelope, where it exerts cell survival functions within many cell types. These functions include ion-conducting channel formation, integral protection from cytotoxic agents, inhibition of proteins that activate caspases responsible for disassembling cells, as well as the ability to modulate cell cycle initiation. Bcl-2 over expression promotes oncogenesis and has been implicated in a variety of high incidence malignancies such as breast, prostate, skin, colon, and pancreas cancer; non-Hodgkin’s lymphoma3 and acute myeloid leukemia (AML). Understanding the biochemical properties of Bcl-2 is essential for improving the treatment of cancer and controlling its mechanisms.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for BCL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BCL-2 present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for BCL-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of BCL-2 bound in the initial step. The color
development is stopped and the intensity of the color is measured. | 
