ELISA法定量测定猪血清、血浆、细胞培养物上清、组织匀浆或其它相关液体中血管紧张素II(ANG II)含量。

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中ANG II水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入ANG II、生物素化的抗猪ANG II抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的ANG II呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

This immunoassay kit allows for the specific measurement of Porcine angiotensin II (ANG II) concentrations in cell culture supernates, tissue homogenates, serum, and plasma.

Angiotensin is an oligopeptide hormone that circulates in the blood and which causes vasoconstriction, increased blood pressure, and release of aldosterone from the adrenal cortex. It is also a powerful dipsogen as a result of its actions on the brain. It is derived from the precursor molecule angiotensinogen, a serum globulin produced in the liver. It plays an important role in the renin-angiotensin system, an endocrine system that helps regulate long-term blood pressure and blood volume in the body. The system can be activated when there is a loss of blood volume or a fall in blood pressure. Angiotensin I is converted to angiotensin II by angiotensin-converting enzyme (ACE), which is found mainly in lung capillaries.

Angiotensin II (ANG II) plays an important role in vascular disease, and angiotensin-converting enzyme inhibitors or Ang II type 1 receptor antagonists can lower blood pressure and reverse vascular remodeling in patients with hypertension or in spontaneously hypertensive rats. Ang II exerts some of its effects on the vasculature by stimulating expression of growth factors including basic fibroblast growth factor, platelet-derived growth factor, TGF , IGF-I and the IGF-I receptor (IGF-IR).

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for ANG II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG II present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for ANG II is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANG II bound in the initial step. The color development is stopped and the intensity of the color is measured.