ELISA法定量测定猪血清、血浆、细胞培养物上清或其它相关液体中VE-cadherin含量。

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中VE-cadherin水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入VE-cadherin抗原、生物素化的抗猪VE-cadherin抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的VE-cadherin呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

This immunoassay kit allows for the specific measurement of porcine VE-cadherin concentrations in cell culture supernates, serum, and plasma.

 

Introduction

Cadherin-5, though member of the family of cadherins has been shown to be functionally as well as structurally distinct from classical cadherins (e.g. E-, N-, P-cadherins). Through its function and location cadherin-5 has been named VE-cadherin. VE-cadherin belongs to the adhesion molecules responsible for cellular interactions. The vascular endothelial cadherin (VE-cadherin) gene encodes a Ca2+-dependent cell adhesion molecule required for the organization of interendothelial junctions. This gene is exclusively and constitutively expressed in endothelial cells. The corresponding protein, an endothelial-specific cadherin, is localized at the intercellular junctions. VE-cadherin mediates homophilic, calcium-dependent aggregation and cell-to-cell adhesion. In addition, it decreases intercellular permeability to high-molecular weight molecules and reduces cell migration rate across a wounded area. Thus, VE-cadherin may exert a relevant role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. The opening of the VE-cadherin mediated endothelial barrier may be a relevant step during neutrophil extravasation. This means that despite the fact that VE-cadherin is a “nonclassical” cadherin by structure, it functions as a classic cadherin.

 

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for VE-cadherin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VE-cadherin present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for VE-cadherin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VE-cadherin bound in the initial step. The color development is stopped and the intensity of the color is measured.