预期应用
ELISA法定量测定猪血清、血浆、细胞培养物上清或其它相关液体中VEGFR-2含量。
实验原理
本试剂盒应用双抗体夹心酶标免疫分析法测定标本中VEGFR-2水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入VEGFR-2抗原、生物素化的抗猪VEGFR-2抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的VEGFR-2呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。
Intended use
This immunoassay kit allows for the specific measurement of porcine VEGFR-2 concentrations in cell culture supernates, serum, and plasma.
Introduction
Vascular endothelial growth factor receptor 2 (VEGFR-2, KDR, Flk-1) is a major receptor transducing VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR-2 undergoes autophosphorylation and becomes activated. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic
domain (Tyr1054/1059). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2. The phosphorylation of Tyr1212 provides a docking site for Grb2 binding, and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCr, as well as Shb. The signaling of VEGFR-2 is necessary for the execution of VEGFstimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for VEGFR-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGFR-2 present is bound by the immobilized antibody. An
enzyme-linked monoclonal antibody specific for VEGFR-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells
and color develops in proportion to the amount of VEGFR-2 bound in the initial step. The color
development is stopped and the intensity of the color is measured. | 
